To qualify as HEPA by US government standards, an air filter must remove (from the air that passes through) 99.97% of particles that have a size of 0.3 µm.

HEPA was commercialized in the 1950s, and the original term became a registered trademark and later a generic term for highly efficient filters.

HEPA filters are composed of a mat of randomly arranged fibres. The fibres are typically composed of fiberglass and possess diameters between 0.5 and 2.0 micrometers. Key factors affecting its functions are fibre diameter, filter thickness, and face velocity. The air space between HEPA filter fibres is typically much greater than 0.3 μm. The common assumption that a HEPA filter acts like a sieve where particles smaller than the largest opening can pass through is incorrect and impractical. Unlike membrane filters at this pore size, where particles as wide as the largest opening or distance between fibres can not pass in between them at all, HEPA filters are designed to target much smaller pollutants and particles. These particles are trapped (they stick to a fibre) through a combination of the following three mechanisms:

Interception

Where particles following a line of flow in the air stream come within one radius of a fibre and adhere to it.

Impaction

Where larger particles are unable to avoid fibres by following the curving contours of the air stream and are forced to embed in one of them directly; this effect increases with diminishing fibre separation and higher air flow velocity.

Diffusion

An enhancing mechanism that is a result of the collision with gas molecules by the smallest particles, especially those below 0.1 µm in diameter, which are thereby impeded and delayed in their path through the filter; this behaviour is similar to Brownian motion and raises the probability that a particle will be stopped by either of the two mechanisms above; this mechanism becomes dominant at lower air flow velocities.

Diffusion predominates below the 0.1 μm diameter particle size. Impaction and interception predominate above 0.4 μm. In between, near the most penetrating particle size (MPPS) 0.21 μm, both diffusion and interception are comparatively inefficient. Because this is the weakest point in the filter's performance, the HEPA specifications use the retention of particles near this size (0.3 μm) to classify the filter. However it is possible for particles smaller than the MPPS to not have filtering efficiency greater than that of the MPPS. This is due to the fact that these particles can act as nucleation sites for mostly condensation and form particles near the MPPS.

Lastly, it is important to note that HEPA filters are designed to arrest very fine particles effectively, but they do not filter out gasses and odor molecules. Circumstances requiring filtration of volatile organic compounds, chemical vapors, cigarette, pet, and/or flatulence odors call for the use of an activated carbon (charcoal) or other type of filter instead of or in addition to a HEPA filter.